Mouse ENOS-3 ELISA Kit

Principle of the assay: eNOS3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-eNOS3 antibody and an eNOS3-HRP conjugate. The assay sample and buffer are incubated together with eNOS3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the eNOS3 concentration since eNOS3 from samples and eNOS3-HRP conjugate compete for the anti-eNOS3 antibody binding site. Since the number of sites is limited, as more sites are occupied by eNOS3 from the sample, fewer sites are left to bind eNOS3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The eNOS3 concentration in each sample is interpolated from this standard curve. Species : Mouse Storage: For 5-7days:Store the whole kit at 4℃For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃. Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: ENOS-3 Uniprot AC: Intended Use: Mouse ENOS-3 ELISA Kitallows for the in vitro quantitative determination of ENOS-3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.