Mouse TBP2 ELISA Kit

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TBP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TBP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TBP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TBP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Species : Mouse Storage: For 5-7days:Store the whole kit at 4℃For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃. Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: TBP2 Uniprot AC: Intended Use: Mouse TBP2 ELISA Kitallows for the in vitro quantitative determination of TBP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.