Mouse TRAP-5a ELISA Kit

Intended Uses: This TRACP5alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TRACP5alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay:TRACP5alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TRACP5alpha antibody and an TRACP5alpha-HRP conjugate. The assay sample and buffer are incubated together with TRACP5alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TRACP5alpha concentration since TRACP5alpha from samples and TRACP5alpha-HRP conjugate compete for the anti-TRACP5alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by TRACP5alpha from the sample, fewer sites are left to bind TRACP5alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRACP5alpha concentration in each sample is interpolated from this standard curve. Species : Mouse Storage: For 5-7days:Store the whole kit at 4℃For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃. Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: TRAP-5a Uniprot AC: Intended Use: Mouse TRAP-5a ELISA Kitallows for the in vitro quantitative determination of TRAP-5a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.