Mouse Wnt3a ELISA Kit

Principle of the Assay:This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Wnt-3a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Wnt-3a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Wnt-3a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Wnt-3a can be calculated. Species : Mouse Storage: Store the whole ELISA kit at 4℃ Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: Wnt3a Uniprot AC: P27467; Intended Use: Mouse Wnt3a ELISA Kitallows for the in vitro quantitative determination of Wnt3a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.