Mouse Sag ELISA Kit

Principle of the Assay: SAG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SAG antibody and an SAG-HRP conjugate. The assay sample and buffer are incubated together with SAG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SAG concentration since SAG from samples and SAG-HRP conjugate compete for the anti-SAG antibody binding site. Since the number of sites is limited, as more sites are occupied by SAG from the sample, fewer sites are left to bind SAG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAG concentration in each sample is interpolated from this standard curve. Species : Mouse Storage: Store the whole ELISA kit at 4℃ Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: Sag Uniprot AC: P20443; Q91W62; Intended Use: Mouse Sag ELISA Kitallows for the in vitro quantitative determination of Sag , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.