Mouse sCTLA4 ELISA Kit

Principle of the Assay: sCTAg4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-sCTAg4 antibody and an sCTAg4-HRP conjugate. The assay sample and buffer are incubated together with sCTAg4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sCTAg4 concentration since sCTAg4 from samples and sCTAg4-HRP conjugate compete for the anti-sCTAg4 antibody binding site. Since the number of sites is limited, as more sites are occupied by sCTAg4 from the sample, fewer sites are left to bind sCTAg4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCTAg4 concentration in each sample is interpolated from this standard curve. Species : Mouse Storage: For 5-7days:Store the whole kit at 4℃For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃. Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: sCTLA4 Uniprot AC: Intended Use: Mouse sCTLA4 ELISA Kitallows for the in vitro quantitative determination of sCTLA4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.