Mouse Clcn2 ELISA Kit

Intended Uses: This CLCN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CLCN2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CLCN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLCN2 antibody and an CLCN2-HRP conjugate. The assay sample and buffer are incubated together with CLCN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLCN2 concentration since CLCN2 from samples and CLCN2-HRP conjugate compete for the anti-CLCN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLCN2 from the sample, fewer sites are left to bind CLCN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLCN2 concentration in each sample is interpolated from this standard curve. Species : Mouse Storage: Store the whole ELISA kit at 4℃ Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: Clcn2 Uniprot AC: Q9R0A1; Q0VBC2; Q9WUJ9; Intended Use: Mouse Clcn2 ELISA Kitallows for the in vitro quantitative determination of Clcn2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.